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© Research
Publication : Emerging infectious diseases

Performance of 2 Commercial Serologic Tests for Diagnosing Zika Virus Infection.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Emerging infectious diseases - 01 Jun 2019

Matheus S, Talla C, Labeau B, de Laval F, Briolant S, Berthelot L, Vray M, Rousset D,

Link to Pubmed [PMID] – 31107211

Link to DOI – 10.3201/eid2506.180361

Emerg Infect Dis 2019 06; 25(6): 1153-1160

Reliable serologic tests are needed for diagnosis and surveillance of Zika virus infection. We evaluated the Euroimmun and Dia.Pro serologic tests for detection of Zika virus IgM and IgG by using a panel of 199 samples from a region endemic for flaviviruses. Kinetics of Zika virus antibodies were monitored from 300 sequential specimens sampled over a period of 10 months after infection. We observed suboptimal performance; sensitivity for Zika virus IgM was low, especially in the Euroimmun assay (49%), whereas IgM could be detected for months with the Dia.pro assay. The specificity of the Zika virus IgG assays was also low, especially that of Dia.Pro (62%); findings were strongly influenced by the epidemiologic context. These results highlight the complexity of serologic diagnosis of Zika virus infection in regions endemic for flaviviruses. Accurate analysis of the performance of assays is required to adapt and interpret algorithms.