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© Research
Publication : Cytometry

Oncosis is associated with exposure of phosphatidylserine residues on the outside layer of the plasma membrane: a reconsideration of the specificity of the annexin V/propidium iodide assay

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cytometry - 01 May 2001

Lecoeur H, Prévost MC, Gougeon ML

Link to Pubmed [PMID] – 11309810

Cytometry 2001 May;44(1):65-72

Following a lethal injury, two modes of cell death can be distinguished, apoptosis and primary necrosis. Cells pass through a prelethal stage characterized by a preservation of membrane integrity, in which they shrink (apoptosis) or swell (oncosis, the early phase of primary necrosis). During apoptosis, a loss of phospholipid asymmetry leads to exposure of phosphatidylserine (PS) residues on the outer leaflet of the plasma membrane. We examined whether the external PS exposure, initially supposed to be specific for apoptosis, was also observed in oncotic cells.