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© Emeline Camand
Marquage par immunofluorescence d'astrocytes tumoraux ou astrocytomes (lignée cellulaire humaine U373), montrant en rouge, APC et en vert, la tubuline des microtubules. APC est un supresseur de tumeur qui est impliqué dans la polarisation des astrocytes normaux. La localisation d'APC est altérée dans des lignées de gliomes. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaire.
Publication : The international journal of biochemistry & cell biology

Nuclear positioning: mechanisms and functions

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The international journal of biochemistry & cell biology - 21 Sep 2011

Dupin I, Etienne-Manneville S

Link to Pubmed [PMID] – 21959251

Int. J. Biochem. Cell Biol. 2011 Dec;43(12):1698-707

The nucleus is the largest organelle in the cell and its position is dynamically controlled in space and time, although the functional significance of this dynamic regulation is not always clear. Nuclear movements are mediated by the cytoskeleton which transmits pushing or pulling forces onto the nuclear envelope. Recent studies have shed light on the mechanisms regulating nuclear positioning inside the cell. While microtubules have been known for a long time to be key players in nuclear positioning, the actin and cytoplasmic intermediate filament cytoskeletons have been implicated in this function more recently and various molecular links between the nuclear envelope and cytoplasmic elements have been identified. In this review, we summarize the recent advances in our understanding of the molecular mechanisms involved in the regulation of nuclear localization in various animal cells and give an overview of the evidence suggesting a crucial role of nuclear positioning in cell polarity and physiology and the consequences of nuclear mispositioning in human pathologies.