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© Research
Publication : Scientific reports

New “haploid biofilm model” unravels IRA2 as a novel regulator of Candida albicans biofilm formation

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Scientific reports - 23 Jul 2015

Seneviratne CJ, Zeng G, Truong T, Sze S, Wong W, Samaranayake L, Chan FY, Wang YM, Wang H, Gao J, Wang Y

Link to Pubmed [PMID] – 26202015

Sci Rep 2015 Jul;5:12433

Clinical isolates of the fungal human pathogen Candida albicans are invariably diploid and heterozygous, impeding genetic study. Recent isolation of C. albicans haploids opens opportunities to apply technologies unfeasible in diploids. However, doubts remain on whether the haploids, derived from chromosome loss, can represent the diploids. Here, we use C. albicans haploids to investigate biofilm, a key virulence attribute. We conducted the first comprehensive characterization of biofilm formation of the haploids in comparison with the diploids. We demonstrate that the haploids form biofilms with essentially the same characteristics as the diploids. Screening a haploid mutant library has uncovered novel GTPase-related genes as biofilm regulators, including IRA2 that encodes an activator of the Ras GTPase. IRA2-deletion mutants develop poorly constructed biofilm in both haploid and diploid C. albicans. Our results demonstrate that the haploids are a valid model for C. albicans biofilm research and a powerful tool for uncovering novel regulators.

https://www.ncbi.nlm.nih.gov/pubmed/26202015