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© Research
Publication : Molecular microbiology

MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri lpa invasins

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular microbiology - 01 Jan 1993

Allaoui A, Sansonetti PJ, Parsot C

Link to Pubmed [PMID] – 8437520

Mol. Microbiol. 1993 Jan;7(1):59-68

The invasive phenotype of Shigella flexneri is conferred by a 220 kb virulence plasmid, pWR100, that encodes both the lpa proteins, which are involved in the entry process, and factors which are required for the export and correct localization of the lpa proteins. We have characterized the mxiD gene, whose expression, like that of the ipa operon, is regulated by temperature. After inactivation of mxiD, the mutant strain was unable to invade HeLa cells and to provoke keratoconjunctivitis in guinea-pigs. Analysis of culture supernatants indicated that wild-type S. flexneri secretes about nine polypeptides and that secretion of several of these, including lpaA, lpaB, and lpaC, is abolished in the mxiD mutant. Examination of the membrane proteins of the wild-type and mxiD strains suggested that MxiD is an outer membrane protein. Amino acid sequence comparison revealed that MxiD is homologous to the YscC protein of Yersinia enterocolitica and to the C-terminal region of the PulD protein of Klebsiella pneumoniae. Both YscC and PulD are involved in extracellular protein secretion. These results indicate that MxiD is an essential component of the lpa secretion apparatus.

http://www.ncbi.nlm.nih.gov/pubmed/8437520