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© Research
Publication : Genesis (New York, N.Y. : 2000)

Mutation studies in ascidians: a review

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Genesis (New York, N.Y. : 2000) - 28 Nov 2014

Crocetta F, Marino R, Cirino P, Macina A, Staiano L, Esposito R, Pezzotti MR, Racioppi C, Toscano F, De Felice E, Locascio A, Ristoratore F, Spagnuolo A, Zanetti L, Branno M, Sordino P

Link to Pubmed [PMID] – 25395385

Genesis 2015 Jan;53(1):160-9

Historically, mutations have had a significant impact on the study of developmental processes and phenotypic evolution. Lesions in DNA are created by artificial methods or detected by natural genetic variation. Random mutations are then ascribed to genetic change by direct sequencing or positional cloning. Tunicate species of the ascidian genus Ciona represent nearly fully realized model systems in which gene function can be investigated in depth. Additionally, tunicates are valuable organisms for the study of naturally occurring mutations due to the capability to exploit genetic variation down to the molecular level. Here, we summarize the available information about how mutations are studied in ascidians with examples of insights that have resulted from these applications. We also describe notions and methodologies that might be useful for the implementation of easy and tight procedures for mutations studies in Ciona.

http://www.ncbi.nlm.nih.gov/pubmed/25395385