Link to Pubmed [PMID] – 29610759
Link to HAL – Click here
Microb Cell 2018 Jan;5(4):169-183
Genetic and molecular studies have indicated that an epigenetic imprint at, the sexual locus of fission yeast, initiates mating type switching. The polar DNA replication ofgenerates an imprint on the Watson strand. The process by which the imprint is formed and maintained through the cell cycle remains unclear. To understand better the mechanism of imprint formation and stability, we characterized the recruitment of early players of mating type switching at theregion. We found that the switch activating protein 1 (Sap1) is preferentially recruited inside theallele on a sequence () that enhances the imprint. The lysine specific demethylases, Lsd1/2, that control the replication fork pause atand the formation of the imprint are specifically drafted inside of, regardless of the allele. The CENP-B homolog, Abp1, is highly enriched next tobut it is not required in the process. Additionally, we established the computational signature of the imprint. Using this signature, we show that both sides of the imprinted molecule are bound by Lsd1/2 and Sap1, suggesting a nucleoprotein protective structure defined as imprintosome.