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© Research
Publication : International journal of molecular medicine

Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in International journal of molecular medicine - 01 Feb 2006

Karst C, Trifonov V, Romanenko SA, Claussen U, Mrasek K, Michel S, Avner P, Liehr T

Link to Pubmed [PMID] – 16391817

Int. J. Mol. Med. 2006 Feb;17(2):209-13

The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.

http://www.ncbi.nlm.nih.gov/pubmed/16391817