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© Research
Publication : Biochimica et biophysica acta

Low molecular weight chitosans: preparation with the aid of papain and characterization

Team: Archived: Aspergillus
Member: Vishu Kumar Aimanianda Bopaiah
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biochimica et biophysica acta - 22 Jan 2004

Vishu Kumar AB, Varadaraj MC, Lalitha RG, Tharanathan RN

Link to Pubmed [PMID] – 14738997

Biochim. Biophys. Acta 2004 Jan;1670(2):137-46

Low molecular weight chitosans (LMWC) of different molecular weight (4.1-5.6 kDa) were obtained by the depolymerization of chitosan using papain (from Carica papaya latex, EC. 3.4.22.2) at optimum conditions of pH 3.5 and 37 degrees C for 1-5 h. Scanning electron microscopy (SEM) showed approximately 15-fold decrease in the particle size after depolymerization. Decrease in the molecular weight was associated with decrease in the degree of acetylation (DA) as evidenced by circular dichroism (CD), FT-IR and solid-state CP-MAS 13C-NMR data. X-ray diffraction pattern revealed slight decrease in the crystallinity index (CrI) whereas the 13C-NMR data showed molecular inhomogeneity. LMWC showed lytic effect towards Bacillus cereus and Escherichia coli more efficiently than native chitosan. The growth inhibitory effect was maximal towards B. cereus, with minimum inhibitory concentration (MIC) of 0.01% (w/v).