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© Research
Publication : Science advances

Longitudinal high-resolution imaging through a flexible intravital imaging window.

Team: Stem Cells And Development
Member: Maria Benavente Diaz
Member: Shahragim Tajbakhsh
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Science advances - 01 Jun 2021

Jacquemin G, Benavente-Diaz M, Djaber S, Bore A, Dangles-Marie V, Surdez D, Tajbakhsh S, Fre S, Lloyd-Lewis B

Link to Pubmed [PMID] – 34134982

Link to DOI – eabg766310.1126/sciadv.abg7663

Sci Adv 2021 Jun; 7(25):

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

https://pubmed.ncbi.nlm.nih.gov/34134982