Link to Pubmed [PMID] – 34134982
Link to HAL – sorbonne-universite-03282426
Link to DOI – 10.1126/sciadv.abg7663
Science Advances , 2021, 7 (25), pp.eabg7663. ⟨10.1126/sciadv.abg7663⟩
Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.