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© Institut Pasteur
Cells infected for 24 hrs with C. Trachomatis. The cell nuclei are labelled in blue, the bacteria appear yellow, within the inclusion lumen. A bacterial protein secreted out the inclusion into the host cytoplasm id labelled in red.
Publication : Methods in molecular biology (Clifton, N.J.)

Labeling of Membrane Complexes for Electron Microscopy

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2017

Gubellini F, Fronzes R

Link to Pubmed [PMID] – 28755367

Methods Mol. Biol. 2017;1635:125-138

Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.

https://www.ncbi.nlm.nih.gov/pubmed/28755367