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© Research
Publication : Journal of virology

Interaction of Theiler’s virus with intermediate filaments of infected cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 01 Dec 1998

Nédellec P, Vicart P, Laurent-Winter C, Martinat C, Prévost MC, Brahic M

Link to Pubmed [PMID] – 9811688

J. Virol. 1998 Dec;72(12):9553-60

Theiler’s murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions between the GDVII and DA strains of Theiler’s virus and BHK-21 host cell proteins in a virus overlay assay. We observed binding of the virions to two proteins of approximately 60 kDa. These proteins were microsequenced and identified as desmin and vimentin, two main components of the intermediate filament network. The association between desmin or vimentin and virions was demonstrated by immunoprecipitation. Anti-desmin and anti-vimentin monoclonal antibodies precipitated GDVII or DA virions from extracts of infected BHK-21 cells. The intracellular distributions of virions and of the desmin and vimentin intermediate filaments of BHK-21 cells were investigated by two-color immunofluorescence confocal microscopy. Following infection, the intermediate filament network was rearranged into a shell-like structure which surrounded a viral inclusion. Finally, close contact between GDVII virus particles and 10-nm intermediate filaments was observed by electron microscopy.

http://www.ncbi.nlm.nih.gov/pubmed/9811688