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© Sandrine Etienne-Manneville
Photo prise à l'avant (dans la protrusion) d'astrocytes primaires de rat en migration. Marquage par immunofluorescence montrant en rouge, p150 Glued, une protéine associée aux extrémités 'plus' des microtubules et en vert la tubuline des microtubules. La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaires.
Publication : Journal of molecular biology

In vitro selection for enzymatic activity: a model study using adenylate cyclase

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of molecular biology - 01 Sep 2003

Strobel H, Ladant D, Jestin JL

Link to Pubmed [PMID] – 12946341

J. Mol. Biol. 2003 Sep;332(1):1-7

An in vitro enzyme selection that can, in principle, be generalised to most chemical reactions, is described. It makes use of filamentous phage display and of a tailor-made antibody fragment directed against the reaction product. The conversion of ATP into 3′,5′-cyclic AMP catalysed by Bordetella pertussis adenylate cyclase is taken as an example.