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© Research
Publication : Molecular immunology

Immunoglobulin VH gene expression following hemorrhage

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular immunology - 01 Sep 1990

Abraham E, Andrade L, Freitas AA

Link to Pubmed [PMID] – 2120578

Mol. Immunol. 1990 Sep;27(9):921-7

Hemorrhage has multiple effects on immunologic response, including alteration of B cell repertoires. In limiting dilution studies, decreased absolute frequencies of splenic clonal precursors specific for bacterial antigens were found after blood loss. In order to better define the effects of hemorrhage on B cell function, we examined immunoglobulin VH gene family expression following blood loss using both in situ hybridization and the RNA colony blot technique. No changes in VH gene family utilization were found after hemorrhage. These results demonstrate that the hemorrhage induced alteration in B cell function involves all VH gene families, without modifying distributive frequencies in VH gene family expression.

http://www.ncbi.nlm.nih.gov/pubmed/2120578