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© Research
Publication : Journal of medical microbiology

Identification of the 33-kDa alkaline protease of Aspergillus fumigatus in vitro and in vivo

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of medical microbiology - 01 Nov 1993

Moutaouakil M, Monod M, Prévost MC, Bouchara JP, Paris S, Latgé JP

Link to Pubmed [PMID] – 8246256

J. Med. Microbiol. 1993 Nov;39(5):393-9

Aspergillus fumigatus produced a 33-kDa serine protease (ALP) in vitro and in vivo. In vitro, this alkaline protease was secreted when the fungus was cultivated in the absence of protein, if the pH of the medium remained close to neutrality. Western blotting and immunoelectronmicroscopy studies showed that ALP was localised in the wall of the fungus and was degraded after secretion in the culture medium under conditions of low pH. Although present in the lung during infection, ALP did not appear to be diagnostically useful and was different from the precipitating chymotrypsin antigen used in the diagnosis of aspergilloma.

http://www.ncbi.nlm.nih.gov/pubmed/8246256