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© Therese Couderc, Marc Lecuit
Publication : Journal of virology

Identical consensus sequence and conserved genomic polymorphism of hepatitis E virus during controlled interspecies transmission

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 28 Mar 2012

Bouquet J, Cheval J, Rogée S, Pavio N, Eloit M

Link to Pubmed [PMID] – 22457521

J. Virol. 2012 Jun;86(11):6238-45

High-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis E virus (HEV) of genotype 3f revealed the same full-length consensus sequence as in the human sample. Twenty-nine percent of polymorphic sites found in HEV from the human sample were conserved throughout the infection of the heterologous host. The interspecies transmission of HEV quasispecies is the result of a genomic negative-selection pressure on random mutations which can be deleterious to the viral population. HEV intrahost nucleotide diversity was found to be in the lower range of other human RNA viruses but correlated with values found for zoonotic viruses. HEV transmission between humans and pigs does not seem to be modulated by host-specific mutations, suggesting that adaptation is mainly regulated by ecological drivers.