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© Institut Pasteur
Culture de myotubes murins infectés par le virus de la rage fixe, observée en immunoflorescence indirecte.
Publication : Virology journal

Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Virology journal - 05 Sep 2006

Hugot JP, Plyusnina A, Herbreteau V, Nemirov K, Laakkonen J, Lundkvist A, Supputamongkol Y, Henttonen H, Plyusnin A

Link to Pubmed [PMID] – 16953877

Virol. J. 2006;3:72

Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.

http://www.ncbi.nlm.nih.gov/pubmed/16953877