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© Research
Publication : Organic & biomolecular chemistry

Generation of long, fully modified, and serum-resistant oligonucleotides by rolling circle amplification

Team: Bioorganic chemistry of nucleic acids
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Organic & biomolecular chemistry - 14 Oct 2015

Hollenstein M

Link to Pubmed [PMID] – 26273951

Org. Biomol. Chem. 2015 Oct;13(38):9820-4

Rolling Circle Amplification (RCA) is an isothermal enzymatic method generating single-stranded DNA products consisting of concatemers containing multiple copies of the reverse complement of the circular template precursor. Little is known on the compatibility of modified nucleoside triphosphates (dN*TPs) with RCA, which would enable the synthesis of long, fully modified ssDNA sequences. Here, dNTPs modified at any position of the scaffold were shown to be compatible with rolling circle amplification, yielding long (>1 kb), and fully modified single-stranded DNA products. This methodology was applied for the generation of long, cytosine-rich synthetic mimics of telomeric DNA. The resulting modified oligonucleotides displayed an improved resistance to fetal bovine serum.