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© Research
Publication : Nature methods

Generating parabiotic zebrafish embryos for cell migration and homing studies

Member: Philippe Herbomel
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature methods - 03 Feb 2013

Demy DL, Ranta Z, Giorgi JM, Gonzalez M, Herbomel P, Kissa K

Link to Pubmed [PMID] – 23377378

Nat. Methods 2013 Mar;10(3):256-8

Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.