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© Research
Publication : The EMBO journal

gal4 transcription activator protein of yeast can function as a repressor in Escherichia coli

Member: Moshe Yaniv
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The EMBO journal - 01 Nov 1987

Paulmier N, Yaniv M, von Wilcken-Bergmann B, Müller-Hill B

Link to Pubmed [PMID] – 3123219

EMBO J. 1987 Nov;6(11):3539-42

The chromosomal lac operator of Escherichia coli was replaced by a 22 bp oligonucleotide containing the binding site of the yeast gal4 protein. Induction of gal4 protein synthesis in these bacteria repressed beta-galactosidase synthesis at least 30-fold. These results show that it is possible to detect in bacteria with a simple assay the DNA binding activity of a eukaryotic protein with a defined sequence specificity. This opens new avenues for the isolation in E. coli of mutants of DNA binding proteins unable to bind to their DNA targets, and for direct cloning in bacteria of cDNA coding for DNA binding proteins with defined sequence specificity.