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© Research
Publication : DNA repair

Fission yeast Swi5 protein, a novel DNA recombination mediator

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in DNA repair - 22 Aug 2007

Haruta N, Akamatsu Y, Tsutsui Y, Kurokawa Y, Murayama Y, Arcangioli B, Iwasaki H

Link to Pubmed [PMID] – 17716957

DNA Repair (Amst.) 2008 Jan;7(1):1-9

The Schizosaccharomyces pombe Swi5 protein forms two distinct protein complexes, Swi5-Sfr1 and Swi5-Swi2, each of which plays an important role in the related but functionally distinct processes of homologous recombination and mating-type switching, respectively. The Swi5-Sfr1 mediator complex has been shown to associate with the two RecA-like recombinases, Rhp51 (spRad51) and Dmc1, and to stimulate in vitro DNA strand exchange reactions mediated by these proteins. Genetic analysis indicates that Swi5-Sfr1 works independently of another mediator complex, Rhp55-Rhp57, during Rhp51-dependent recombinational repair. In addition, mutations affecting the two mediators generate distinct repair spectra of HO endonuclease-induced DNA double strand breaks, suggesting that these recombination mediators differently regulate recombination outcomes in an independent manner.

http://www.ncbi.nlm.nih.gov/pubmed/17716957