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© Michel Huerre
Coupe histologique de foie de patient atteint d'une hépatite C chronique active avec infiltrat (opacité) folliculaire (lymphocytes) (Grossissement X 400). L'hépatite C chronique peut être à l'origine de cirrhose et cancer du foie
Publication : Journal of virological methods

Expression of the gp150 maedi visna virus envelope precursor protein by mammalian expression vectors

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virological methods - 01 Aug 2007

Fraisier C, Arnarson H, Barbezange C, Andrésdŏttir V, Carrozza ML, De Andrés D, Tolari F, Rosati S, Luján L, Pépin M, Amorena B, Harkiss G, Blacklaws B, Suzan-Monti M

Link to Pubmed [PMID] – 17675253

J. Virol. Methods 2007 Dec;146(1-2):363-7

There are very few previous reports of expression of native full-length maedi visna virus (MVV) Env gp150 protein in the literature. Therefore the use of different plasmid and viral expression vectors to obtain full-length gp150 was investigated. A mammalian expression plasmid, pN3-Env, was constructed containing the MVV env gene encoding the precursor protein gp150 Env. The functionality of the recombinant plasmid was tested for expression in HEK293 cells. A recombinant modified vaccinia Ankara virus, MVA-Env, with expression detected in avian cells was also made. The expression of the MVV gp150 Env precursor protein was shown for the first time upon transfection of the eukaryotic HEK293 cells by the pN3-Env plasmid DNA as demonstrated by Western blot analysis. These plasmid or viral expression vectors are of potential use in MVV vaccines.

http://www.ncbi.nlm.nih.gov/pubmed/17675253