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© Research
Publication : Journal of virology

Expression of a membrane-anchored glycoprotein, the influenza virus hemagglutinin, by dicistronic replicons derived from the poliovirus genome

Member: Sylvie van Der Werf
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 01 May 2002

Vignuzzi M, Gerbaud S, van der Werf S, Escriou N

Link to Pubmed [PMID] – 11967344

J. Virol. 2002 May;76(10):5285-90

Mono- and dicistronic poliovirus replicons were constructed to express the influenza virus hemagglutinin, retaining its signal peptide and transmembrane region. Picornavirus genomes do not normally encode glycoproteins, and only the dicistronic replicon, in which the foreign and poliovirus sequences were separated by the encephalomyocarditis virus internal ribosomal entry site, replicated and expressed glycosylated hemagglutinin.