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© Research
Publication : PloS one

Ex vivo cytosolic delivery of functional macromolecules to immune cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PloS one - 13 Apr 2015

Sharei A, Trifonova R, Jhunjhunwala S, Hartoularos GC, Eyerman AT, Lytton-Jean A, Angin M, Sharma S, Poceviciute R, Mao S, Heimann M, Liu S, Talkar T, Khan OF, Addo M, von Andrian UH, Anderson DG, Langer R, Lieberman J, Jensen KF

Link to Pubmed [PMID] – 25875117

PLoS ONE 2015;10(4):e0118803

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.

http://www.ncbi.nlm.nih.gov/pubmed/25875117