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© Research
Publication : The EMBO journal

Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The EMBO journal - 01 Apr 2002

Guichet A, Wucherpfennig T, Dudu V, Etter S, Wilsch-Bräuniger M, Hellwig A, González-Gaitán M, Huttner WB, Schmidt AA

Link to Pubmed [PMID] – 11927550

EMBO J. 2002 Apr;21(7):1661-72

We characterized Drosophila endophilin A (D-endoA), and generated and analysed D-endoA mutants. Like its mammalian homologue, D-endoA exhibits lysophosphatidic acid acyl transferase activity and contains a functional SH3 domain. D-endoA is recruited to the sites of endocytosis, as revealed by immunocytochemistry of the neuromuscular junction (NMJ) of mutant L3 larvae carrying the temperature-sensitive allele of dynamin, shibire. D-endoA null mutants show severe defects in motility and die at the early L2 larval stage. Mutants with reduced D-endoA levels exhibit a range of defects of synaptic vesicle endocytosis, as observed at L3 larvae NMJs using FM1-43 uptake and electron microscopy. NMJs with an almost complete loss of synaptic vesicles did not show an accumulation of intermediates of the budding process, whereas NMJs with only slightly reduced levels of synaptic vesicles showed a striking increase in early-stage, but not late-stage, budding intermediates at the plasma membrane. Together with results of previous studies, these observations indicate that endophilin A is essential for synaptic vesicle endocytosis, being required from the onset of budding until fission.

http://www.ncbi.nlm.nih.gov/pubmed/11927550