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© Research
Publication : International immunology

Endogenous VH gene family expression in immunoglobulin-transgenic mice: evidence for selection of antibody repertoires

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in International immunology - 01 Jan 1991

Grandien A, Coutinho A, Andersson J, Freitas AA

Link to Pubmed [PMID] – 2049337

Int. Immunol. 1991 Jan;3(1):67-73

VH gene family expression in single cells of the emergent, available and actual B cell repertoires of C57BL/6 mice was compared to that of two immunoglobulin (Ig)-transgenic B6 lines (B6-Sp6 and M54). We found that less than 5% of bone marrow cells of transgenic mice express endogenous VH genes and that the vast majority (95%) of the peripheral, mature B cell repertoire in these animals is composed of cells expressing the VHJ558 transgenic family. Unimmunized transgenic mice, however, diversify VH gene family usage by ‘background’ Ig-secreting cells in the spleen, greater than 50% of which express endogenous VH genes. The pattern of endogenous VH gene family expression in the actual repertoire of B6-Sp6 mice is indistinguishable from that of normal B6 mice. In contrast, actual repertoires of M54 mice differ by a 4- to 5-fold higher representation of the VHQ52 family. These results demonstrate a powerful positive selection of B cells into the secretory compartments of unimmunized animals, show that actual and available repertoires differ very markedly, and suggest that V region interactions participate in the selection of ‘natural antibody’ repertoires.

http://www.ncbi.nlm.nih.gov/pubmed/2049337