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© Research
Publication : Aviation, space, and environmental medicine

Effects of hypergravity on lung carcinoma cells maintained in continuous organotypic culture

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Aviation, space, and environmental medicine - 01 Nov 1990

Croute F, Gaubin Y, Prevost MC, Beaupain R, Pianezzi B, Soleilhavoup JP

Link to Pubmed [PMID] – 2256871

Aviat Space Environ Med 1990 Nov;61(11):1002-6

The effects of hypergravity levels ranging from 1 to 15 g were studied on A549 lung adenocarcinoma cell line, cultivated as nodules. This organotypic culture model preserves as closely as possible the cellular structures and differentiation functions of the in vivo situation. Nodules submitted to hypergravity conditions for 27 d did not show any change of cell growth, protein and DNA contents, compared with controls. Also, cellular differentiation, as regards intracellular phospholipid composition and more particularly phosphatidylcholine content, appeared undisturbed. The only obvious effect of hypergravity was a modification of the structural organization, with a disappearance of the large alveoli present at the surrounding of control nodules and the development of a dense cellular mass instead.

http://www.ncbi.nlm.nih.gov/pubmed/2256871