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© Institut Pasteur
Phlébotome. Petit diptère de la famille des psychodidés (dans l'Ancien Monde). La femelle hématophage peut être vectrice entre autres de leishmaniose qu'elle transmet par piqûre à un vertébré, chien (hôte principal réservoir), homme ou encore rat (hôtes occasionnels).
Publication : Methods in molecular biology (Clifton, N.J.)

dsRNA uptake in adult Drosophila

Team: Viruses and RNA Interference
Member: Carla Saleh
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2011

Obadia B, Saleh MC

Link to Pubmed [PMID] – 21431691

Methods Mol. Biol. 2011;721:253-63

RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans. In this chapter, we describe how to introduce exogenous dsRNA into adult Drosophila in order to interfere with endogenous or viral gene expression.