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© Research
Publication : Data in brief

Data on antigen recognition hindrance by antibodies covalently immobilized to Protein G magnetic beads by dimethyl pimelimidate (DMP) cross-linking

Team: Mass Spectrometry for Biology
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Data in brief - 21 Dec 2018

Caminha MA, de Lorena VMB, de Oliveira Júnior W, Perales J, Carvalho PC, Lima DB, Cavalcanti MDGAM, Martins SM, Valente RH, Menna-Barreto RFS

Link to Pubmed [PMID] – 30671503

Data Brief 2019 Feb;22:516-521

The data presented herein is related to the article entitled ” immunoproteome: calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy” [1]. Electrophoretic analyses under denaturing and reducing conditions indicate that covalent immobilization of human IgG to Protein G magnetic beads by cross-linking with 50 mM dimethyl pimelimidate hinders the recognition of antigens in immunoprecipitation assays.