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© A-M. Pais-Correia, M-I. Thoulouze, A. Alcover, A. Gessain
Mise en évidence de structures de type "biofilm ", formées par le rétrovirus HTLV-1 générés par des cellules infectées (cellules du haut), qui ont été transmis à un autre lymphocyte (cellule du bas). Micrographie en microscopie électronique à balayage. Image colorisée.
Publication : Journal of virology

Construction and characterization of a human T-cell lymphotropic virus type 3 infectious molecular clone

Team: Oncogenic Virus Epidemiology and Pathophysiology
Member: Antoine Gessain
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 16 Apr 2008

Chevalier SA, Ko NL, Calattini S, Mallet A, Prévost MC, Kehn K, Brady JN, Kashanchi F, Gessain A, Mahieux R

Link to Pubmed [PMID] – 18417569

J. Virol. 2008 Jul;82(13):6747-52

We and others have uncovered the existence of human T-cell lymphotropic virus type 3 (HTLV-3). We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro, and tax/rex as well as minus-strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24(gag) protein is detected in the cell culture supernatant. Transfection of 293T-long terminal repeat (LTR)-green fluorescent protein (GFP) cells with the HTLV-3 clone promotes formation of syncytia, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.