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© Research
Publication : Journal of bacteriology

Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli

Team: Group : Patrick Trieu-Cuot
Member: Patrick Trieu-Cuot
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of bacteriology - 01 Sep 1988

Trieu-Cuot P, Carlier C, Courvalin P

Link to Pubmed [PMID] – 3137216

J. Bacteriol. 1988 Sep;170(9):4388-91

The possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pBR322-pAM beta 1 chimeric plasmid, designated pAT191. This shuttle vector, which possesses the tra functions of the streptococcal plasmid pAM beta 1, was conjugatively transferred from Enterococcus faecalis to Escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.