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© Research
Publication : Molecular and cellular probes

Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus

Team: Archived: International Group for Data Analysis
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular and cellular probes - 01 Jun 1989

Sakamoto H, Lemaire O, Merdinoglu D, Guesdon JL

Link to Pubmed [PMID] – 2671680

Mol. Cell. Probes 1989 Jun;3(2):159-66

Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.