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© Michel-Robert Popoff
Clostridium difficile en microscopie à contraste de phase. On distingue des bactéries sporulées, non sporulées et d'autres en cours de lyse (destruction). Bactérie de l'environnement (sol, eau, foin, sable), elle est à l'origine d'infections nosocomiales survenant après un traitement antibiotique : Clostridium difficile prédomine alors que les autres bactéries de la flore intestinale ont été détruites. L'infection peut provoquer deux types de pathologies graves : les colites pseudo-membraneuses dont l'origine est quasiment due à 100 % à C. difficile et la diarrhée post-antibiothérapie due à C. difficile dans 30 % des cas de ces diarrhées.
Publication : Bioorganicheskaia khimiia

[Cloning and determining the primary structure of fragments of the RNA-polymerase beta-subunit genes from representatives of Baikal picoplankton]

Team: Archived: Olga Soutourina
Member:
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Published in Bioorganicheskaia khimiia - 01 Dec 1998

Suturina OA, Suturina IuA, Semenova EA

Link to Pubmed [PMID] – 10079948

Bioorg. Khim. 1998 Dec;24(12):906-9

Fragments of the RNA polymerase beta-subunit genes from representatives of picoplankton of Lake Baikal were cloned using polymerase chain reaction (PCR), and the resulting recombinant clones were sequenced. An analysis of 93 clones revealed 40 different sequences. A comparison of these nucleotide sequences and the corresponding amino acid sequences deduced with their homologues from databanks confirmed the high conservation of the RNA polymerase beta-subunit region involved in the formation of the enzyme active site.