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© Research
Publication : Genome research

Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Genome research - 12 Nov 2003

Sousa-Nunes R, Rana AA, Kettleborough R, Brickman JM, Clements M, Forrest A, Grimmond S, Avner P, Smith JC, Dunwoodie SL, Beddington RS

Link to Pubmed [PMID] – 14613977

Genome Res. 2003 Dec;13(12):2609-20

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

http://www.ncbi.nlm.nih.gov/pubmed/14613977