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© Research
Publication : Molecular immunology

Characterization of monoclonal antibodies against prostaglandin E2: fine specificity and neutralization of biological effects

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular immunology - 01 Mar 1985

David F, Sommé G, Provost-Wisner A, Roth C, Astoin M, Dray F, Thèze J

Link to Pubmed [PMID] – 3858663

Mol. Immunol. 1985 Mar;22(3):339-46

The specificity and heterogeneity of the immune response of BALB/c mice immunized with prostaglandin E2 (PGE2) coupled to thyroglobulin was studied. All the animals (n = 50) responded to PGB2, a transformation product of PGE2. However, following repeated injections most of the animals (n = 30) were also able to respond to PGE2. Cellular hybridizations were performed and five anti-PGE2 monoclonal antibodies were isolated and analysed. They are mainly directed against the ring and the omega-chain of PGE2 but their specificity toward the alpha-chain is more limited. The association constants are greater than to 1 X 10(9) M-1. The monoclonal antibody 8E.57.71 (Ka = 1.3 X 10(10) M-1) is particularly convenient for sensitive radioimmunoassays (detection limit 25pg/ml, when iodinated tracer is used). Anti-PGE2 monoclonal antibodies were found to neutralize the specific binding of [3H]PGE2 to rat brain hypothalamic receptors and to inhibit the PGE2 induction of rat fundus muscular contraction.

http://www.ncbi.nlm.nih.gov/pubmed/3858663