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© Institut Pasteur
Macrophage apparaissant derrière un lymphocyte. Image colorisée.
Publication : Journal of receptor and signal transduction research

Binding of human GM-CSF to synthetic peptides of the alpha subunit of its receptor

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of receptor and signal transduction research - 01 Jan 1996

Di Bartolo V, Danè A, Cassano E, Viganò S, Chiello E, Verniani D, Beffy P, Pegoraro S, Hamdan M, Rovero P, Revoltella RP

Link to Pubmed [PMID] – 8771532

J. Recept. Signal Transduct. Res. 1996 Jan-Mar;16(1-2):77-92

We have synthesized a series of peptides corresponding to portions of the extracellular domain of human granulocyte-macrophage colony stimulating factor receptor alpha subunit (hGM-CSFR alpha). The sequences were chosen according to the homology between hGM-CSFR alpha and the growth hormone receptor (GHR) and correspond to the regions reported to form the binding site of the latter receptor. The peptides were examined for their binding activity to hGM-CSF by affinity chromatography on resin-immobilized hGM-CSF and by a solid phase binding assay. For peptides endowed with hGM-CSF binding activity were identified and the postulated homology between the binding sites of hGM-CSFR alpha GHR was confirmed.

http://www.ncbi.nlm.nih.gov/pubmed/8771532