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© Research
Publication : Development (Cambridge, England)

An in situ assessment of the routes and extents of colonisation of the mouse embryo by embryonic stem cells and their descendants

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Development (Cambridge, England) - 01 Dec 1990

Lallemand Y, Brûlet P

Link to Pubmed [PMID] – 2100261

Development 1990 Dec;110(4):1241-8

An embryonic stem (ES) cell line stably expressing lacZ under the control of an endogenous promoter has been isolated and used as a marker to follow the fate of ES cells injected into blastocysts and morulae, before midgestation. The results show a multisite pattern of blastocyst colonization by ES cells deposited into the blastocoel cavity and a low degree of mingling between ES cells and ICM cells. Furthermore, analysis of dispersal of ES cell descendants in postimplantation chimaeric embryos showed that colonization can be highly variable from one region of the embryo to another. In contrast, a high and reproducible degree of chimaerism was obtained when the ES cells were injected at the morula stage prior to ICM formation.

http://www.ncbi.nlm.nih.gov/pubmed/2100261