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© Research
Publication : Methods in enzymology

A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in enzymology - 24 Feb 2018

Piazza A, Koszul R, Heyer WD

Link to Pubmed [PMID] – 29523235

Meth. Enzymol. 2018;601:27-44

Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3′-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.

https://www.ncbi.nlm.nih.gov/pubmed/29523235