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© Research
Publication : Clinical chemistry

A novel real-time duplex PCR assay for detecting penA and ponA genotypes in Neisseria gonorrhoeae: Comparison with phenotypes determined by the E-test

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Clinical chemistry - 19 Oct 2006

Vernel-Pauillac F, Merien F

Link to Pubmed [PMID] – 17053151

Clin. Chem. 2006 Dec;52(12):2294-6

For many years, the pathogenic bacterium Neisseria gonorrhoeae, the etiologic agent of gonorrhea, was generally susceptible to penicillin, until the emergence of resistant strains. Well-characterized genetic variations in the penicillin resistance-determining region correlate with decreased susceptibility to penicillin. At least 5 genes (penA, penB, mtrR, ponA, and penC) are involved in the chromosomally mediated resistance to this antibiotic. To date, no development of multiplex PCR assays targeting a range of gonococcal genes and variations as a means of predicting antibiotic resistance has been reported.

http://www.ncbi.nlm.nih.gov/pubmed/17053151