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© Research
Publication : Molecular microbiology

A novel lysis system in PM2, a lipid-containing marine double-stranded DNA bacteriophage

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular microbiology - 01 Jun 2007

Krupovic M, Daugelavicius R, Bamford DH

Link to Pubmed [PMID] – 17555443

Mol. Microbiol. 2007 Jun;64(6):1635-48

In this study we investigated the lysis system of the lipid-containing double-stranded DNA bacteriophage PM2 infecting Gram-negative marine Pseudoalteromonas species. We analysed wt and lysis-deficient phage-induced changes in the host physiology and ascribed functions to two PM2 gene products (gp) involved in lysis. We show that bacteriophage PM2 uses a novel system to disrupt the infected cell. The novelty is based on the following findings: (i) gp k is needed for the permeabilization of the cytoplasmic membrane and appears to play the role of a typical holin. However, its unique primary structure [53 aa, 1 transmembrane domain (TMD)] places it into a new class of holins. (ii) We have proposed that, unlike other bacteriophages studied, PM2 relies on lytic factors of the cellular origin for digestion of the peptidoglycan. (iii) gp l (51 aa, no TMDs) is needed for disruption of the outer membrane, which is highly rigidified by the divalent cations abundant in the marine environment. The gp l has no precedent in other phage lytic systems studied so far. However, the presence of open reading frame l-like genes in genomes of other bacterial viruses suggests that the same system might be used by other phages and is not unique to PM2.

http://www.ncbi.nlm.nih.gov/pubmed/17555443