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Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-Sce I

Synthesis and enzymatic polymerisation of 5-amino-1-(2′-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide-5′- triphosphate

© Mélanie Falord, Tarek Msadek, Jean-Marc Panaud

Staphylococcus aureus "golden staph" in scanning electron microscopy.

Publication : DNA sequence : the journal of DNA sequencing and mapping

A gene encoding a tyrosine tRNA synthetase is located near sacS in Bacillus subtilis

Team: Biology of Gram-Positive Pathogens

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Diseases

Organisms

Applications

Technique

Published in DNA sequence : the journal of DNA sequencing and mapping - 01 Jan 1991

Glaser P, Kunst F, Débarbouillé M, Vertès A, Danchin A, Dedonder R

Link to Pubmed [PMID] – 1806041

DNA Seq. 1991;1(4):251-61

Within the frame of an attempt to sequence the whole Bacillus subtilis genome, a region of 5.5 kbp of the B. subtilis chromosome near the sacS locus has been sequenced. It contains five complete coding sequences, including the sequence of sacY, three unknown CDS and a sequence coding for a tyrosine tRNA synthetase. That the corresponding CDS encodes a functional synthetase has been demonstrated by complementation of an Escherichia coli mutant possessing a thermosensitive tRNA synthetase. Insertion of a kanamycin resistance cassette in the B. subtilis chromosome at the corresponding locus resulted, however, in no apparent phenotype, demonstrating that this synthetase is dispensable. Finally phylogenetic relationships between known tyrosine and tryptophan tRNA synthetases are discussed.

Published on: 01 Jan 1991