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© Christine Schmitt, Meriem El Ghachi, Jean-Marc Panaud
Bactérie Helicobacter pylori en microscopie électronique à balayage. Agent causal de pathologies de l'estomac : elle est responsable des gastrites chroniques, d'ulcères gastriques et duodénaux et elle joue un rôle important dans la genèse des cancers gastriques (adénocarcinomes et lymphomes).
Publication : Archives of biochemistry and biophysics

A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid’s proteome

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Archives of biochemistry and biophysics - 23 Sep 2017

Trindade F, Bastos P, Leite-Moreira A, Manadas B, Ferreira R, Soares SF, Daniel-da-Silva AL, Falcão-Pires I, Vitorino R

Link to Pubmed [PMID] – 28951296

Arch. Biochem. Biophys. 2017 11;634:1-10

Owing to their close proximity, pericardial fluid (PF)’s proteome may mirror the pathophysiological status of the heart. Despite this diagnosis potential, the knowledge of PF’s proteome is scarce. Large amounts of albumin hamper the characterization of the least abundant proteins in PF. Aiming to expand PF’s proteome and to validate the technique for future applications, we have fractionated and characterized the PF, using N-(trimethoxysilylpropyl)ethylenediamine triacetic acid (EDTA)-functionalized magnetic nanoparticles (NPs@EDTA) followed by a GeLC-MS/MS approach. Similarly to an albumin-depletion kit, NPs@EDTA-based fractionation was efficient in removing albumin. Both methods displayed comparable inter-individual variability, but NPs@EDTA outperformed the former with regard to the protein dynamic range as well as to the monitoring of biological processes. Overall, 565 proteins were identified, of which 297 (>50%) have never been assigned to PF. Moreover, owing to this method’s good proteome reproducibility, affordability, rapid automation and high binding ability of NP@EDTA, it bears a great potential towards future clinical application.

https://www.ncbi.nlm.nih.gov/pubmed/28951296