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© Research
Publication : Nature methods

Sharper low-power STED nanoscopy by time gating

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature methods - 05 Jun 2011

Vicidomini G, Moneron G, Han KY, Westphal V, Ta H, Reuss M, Engelhardt J, Eggeling C, Hell SW

Link to Pubmed [PMID] – 21642963

Nat. Methods 2011 Jul;8(7):571-3

Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

http://www.ncbi.nlm.nih.gov/pubmed/21642963