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© Nicola Festuccia, Institut Pasteur
Mouse embryonic stem cells stained for Ki67 (red) and Esrrb (green)
Publication : Stem cell reports

2i, or Not 2i: The Soliloquy of Nanog-Negative Mouse Embryonic Stem Cells

Team: Epigenomics, Proliferation and the Identity of Cells – EPIC
Member: Pablo Navarro Gil
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Stem cell reports - 10 Jul 2018

Navarro P

Link to Pubmed [PMID] – 29996086

Stem Cell Reports 2018 07;11(1):1-3

In this issue of Stem Cell Reports, Hastreiter et al. (2018) use continuous time-lapse imaging of mouse embryonic stem cells to investigate how the inhibition of GSK3b and MEK/ERK (2i) leads to homogeneous expression of the transcription factor Nanog. They show that both induction of Nanog expression and selection against cells expressing low levels of Nanog contribute to the homogeneous appearance of 2i cultures.