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Birth date : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

Date de naissance  : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

date de naissance : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

Date de naissance  : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

Birth date : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

Date de naissance  : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

date de naissance : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)

Date de naissance  : 11/4/1987
Nationality : Argentinean

Education :

  • 2/2016 : PhD National University of La Plata, Exact Sciences Faculty – Argentina
  • 12/2010 : Degree in Biochemistry of National University of La Plata, Exact Sciences Faculty – Argentina

Research experiences :

Since 4/2016 : Postdoctoral position contract with Université Paris Diderot, Paris 7, in Unité Lymphopoiesis – Institut Pasteur, Paris

4/2011 – 2/2016 : PhD at National University of La Plata, Exact Sciences Faculty – Argentina entitled “Analysis and use of therapeutic strategies for modulation of inflammatory response in intestinal pathologies” under supervision of Dr. Guillermo Docena 

since 2011 :  Teaching assistant in Biochemistry and Molecular Biology. School of Medicine, UNLP – Argentina

7/2010 – 12/2010 : internship “Sor María Ludivica” Pediatrics Hospital of La Plata. Laboratory of Immunology

Fellowships & grants:

  • April 2014 – March2016  : CONICET (National Council of Science and Technology) .
  • 2014 -2017 : National University of La Plata. Project: “Development of immunotherapy models for the treatment of food allergies”. As PhD student.

  • 2013-2016 : National Agency for Promotion Science and Technology. Project: “Immunotherapies design for food allergies treatment”.  As PhD student.

  • 2011-2014 : National Agency for Promotion Science and Technology. Project: “Gastrointestinal mucosa cell populations balance in homeostasis and inflammation”. As PhD student.

  • April 2011 – March 2014 : ANPCyT (National Agency for Promotion Science and Technology)

Publications :
Paola Lorena Smaldini; María Lucía Orsini Delgado; Carlos Alberto Fossati; Guillermo Horacio Docena. Orally-induced intestinal CD4+ CD25+ FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampen Food Allergic Reactions. PlosOne.2015. Oct 30;10(10):e0141116. doi: 10.1371/journal.pone.0141116. eCollection 2015.

Cecilia I, Muglia; Rodrigo Papa Gobbi; Paola Smaldini;María Lucía Orsini Delgado; Martín Candia; Carolina Zanuzzi; Alicia Sambuelli; Andrés Rocca; Marta A. Toscano; Gabriel A. Rabinovich; Guillermo H. Inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1. Journal of cell physiology.2016 Jul;231(7):1575-85. doi: 10.1002/jcp.25249. Epub 2015 Dec 28.

Courses :

  • Statistics and experimental designs with laboratory animals (2012). Duration 40h. University of Buenos Aires. Exam: approved.
  • Introduction to science philosophy (2012).Duration: 48h. School of Sciences, UNLP. Exam: approved
  • Flow Cytometry course (2012). Duration 40 h. “Rioplatense” group of flow cytometry. Buenos Aires, Argentina. Exam: approved.
  • New concepts of programmed cell death (2013). Duration 50hs. Biology and experimental medicine institute (IBYME), CONICET. Buenos Aires, Argentina. Exam: approved.

Attendance to congresses :

  • 2009.National Biochemistry Congress CUBRA X. Buenos Aires, Argentina.
  • 2011. LIX Annual Meeting of the Argentinean Society of Immunology. Tucumán, Argentina
  • 2012. LX Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2013.LXI Annual Meeting of the Argentinean Society of Immunology. Córdoba, Argentina.
  • 2014. LXII Annual Meeting of the Argentinean Society of Immunology. Buenos Aires, Argentina
  • 2015. LXIII Annual Meeting of the Argentinean Society of Immunology. II French-Argentinean Congress of Immunology. Ciudad Autónoma Buenos Aires, Argentina.

Works submitted to national congresses :
201
1.Efecto inmunomodulatorio in vivo que bacterias Actinomicetales ejercen en inflamación intestinal.(In vivo immunomodulatory effect of Actinomycetales in intestinal inflammation).Orsini Delgado, M. Lucía; Smaldini, Paola L.; Stanford, John; Hiriart Yanina; Fossati, Carlos A.; Docena, Guillermo H.LIX Annual Meeting of the Argentinean Society of Immunology.

2011.Reactividad Cruzada: Inmunoterapia empleando proteínas de soja en un modelo murino de alergia a leche de vaca. (Cross reactivity: use of soybean proteins as immunotherapy in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LIX Annual Meeting of the Argentinean Society of Immunology.

2012. Identificación de linfocitos T regulatorios productores de IL-10 como mediadores de la tolerancia oral inducida en un modelo murino de alergia a la leche de vaca.(Identification of IL-10 producing Treg cells as mediators of induced oral tolerance in a cow’s milk allergy mouse model). Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A.; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012.Empleo de alergenos de realctividad cruzada en inmunoterapia oral para la modulación de la respuesta alérgica en un modelo murino de alergia a la leche bovina. (Use of cross reactivity allergens in oral immunotherapy for modulation of allergic response in a cow’s milk allergy mouse model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2012. Optimización de las condiciones experimentales de cultivos primarios de enterocitos murinos. (Optimization of experimental conditions for mouse intestinal epithelial cells primary culture). Di Claudio, Fiorella; Orsini Delgado, M. Lucía; Grigera, José Raúl; Muglia, Cecilia; Docena, Guillermo H. LX Annual Meeting of the Argentinean Society of Immunology.

2013. Oral immunotherapy for food allergy in a cow’s milk allergy mouse model. Orsini Delgado, M. Lucía; Smaldini, Paola L.; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013. Development of sublingual immunotherapy in a cow’s milk allergy mouse model. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2013.Development of allergen-specific immunotherapy based on the biological cross- reactivity between cow’s milk and soybean. Candreva, Angela M; Curciarello, Renata; Smaldini, Paola L.; Orsini Delgado, M. Lucía; Petruccelli, Silvana; Docena, Guillermo H. LXI Annual Meeting of the Argentinean Society of Immunology.

2014. Pro-inflammatory effect of volcanic ash in a colitis model. Orsini Delgado, M. Lucía; Papa Gobbi, Rodrigo; Sambuelli, Alicia; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2014. Orally induced CD4+CD25+Foxp3+ Treg supressed food allergic reactions.Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. LXII Annual Meeting of the Argentinean Society of Immunology.

2015. Inmunoterapia oral versus sublingual en un modelo experimental de alergia alimentaria. Células y moléculas involucrad (Oral vs sublingual immunotherapy in a food allergy experimental model). Smaldini, Paola L.; Orsini Delgado, M. Lucía; Fossati, Carlos A.; Docena, Guillermo H. Ciudad Autónoma de Buenos Aires, Argentina. XXXVIII Anual Congress of the Argentinean Asossiation of Allergy and Clinical Immunology.

Works submitted to international congresses :
2
012. Oral Immunotherapy using soy proteins modulates the allergic response in a mouse model of cow’s milk allergy. Smaldini, Paola L.; Orsini Delgado, M. Lucía; Curciarello, Renata; Candreva, Angela M; Fossati, Carlos A; Docena, Guillermo Lima, Peru. ImmunoPeru 2012, 10th Congress of the Latin American Association of Immunology.

2015. Pro-inflammatory effect of volcanic ash (va) in a colitis model – is the va linked with the ibd epidemiology? Orsini Delgado, M. Lucía; Sambuelli, Alicia; Papa Gobbi, Rodrigo; Gil, Anibal; Negreira, Silvia; Huernos, Sergio; Goncalves, Silvina; Bellicoso, Maricel; Chavero, Paula; Tirado, Pablo; Docena, Guillermo Barcelona, Spain. 10th congress of European Crohn’s and Colitis Organization (ECCO).

2015. Use of Omp16 as a mucosal adjuvant in a sublingual immunotherapy for food all Orsini Delgado, M. Lucía; Smaldini, Paola L.; Pasquevich, Karina; Cassataro, Juliana; Docena, Guillermo H. Medellín, Colombia. ImmunoColombia 2015, 11th   Congress of the Latin American Association of Immunology.

Professional affiliations :
Adherent Member of the Argentinean Society of Immunology.

Languages :
Spanish: Native speaker.
English: First Certificate (2003).
French: Basic knowledge.
Portuguese: Basic knowledge.

Technical lab skills :

  • Animal models (food allergy mouse model and colitis mouse model): Different in vivo assays and administration routes (intragastric, intrarectal, sublingual, subcutaneous, intravenous, intraperitoneal, intranasal) and blood sampling.
  • Primary culture of cells isolated from spleen, mesenteric lymph nodes, sublingual lymph nodes and lamina propria.
  • Cell lines culture. Multicolor flow cytometry.
  • Immunoassays: ELISA, EAST, Immunoblotting
  • Protein isolation. Molecular biology.
  • Histology (H&E staining, immunofluorescence)