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© Institut Pasteur
Macrophages et lymphocytes de souris. Image colorisée.
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CV

EDUCATION : Johns Hopkins University

  •  M.S. in Biotechnology student, expected graduation May 2018

Case Western Reserve University

  • A. Evolutionary Biology & Political Science with minor in Russian Language, graduated in 2012
  • Senior Political Science thesis: How NIH Grants are a Stimulus for Local Economies
  • Winner of 2008 Common Reading Essay Contest

RELATED SKILLS :

●       Molecular Biology/Biochemistry lab techniques ●       Bioinformatics Software ●       Cell Culture
●       Animal Surgery and Research ●       Lab Management and Organization ●       Animal Husbandry (mice)

PUBLICATIONS :

  • Otegbeye, Mackowski, Ojo, Lima, & Wald. (2015). The IL-15 Super-Agonist ALT-803 Promotes Superior Activation and Cytotoxicity of Ex Vivo Expanded NK Cells Against AML. Blood,126(23), 3090. http://www.bloodjournal.org/content/126/23/3090?sso-checked=true
  • Otegbeye, F., Mackowski, N., Ojo, E., Moreton, S., Lima, M. D., & Wald, D. (2016). Enhancing the Functional Activity of Natural Killer Cells Against Myeloid Leukemia with the IL-15 Super-Agonist, ALT-803. Biology of Blood and Marrow Transplantation, 22(3). doi:10.1016/j.bbmt.2015.11.485, http://www.bbmt.org/article/S1083-8791(15)01242-2/pdf
  • Otegbeye, F., Ojo, E., Mackowski, N., Moreton, S., & Wald, D. (2016). Inhibiting TGF-Beta Signaling in the Tumor Micro-Environment Enhances the Cytotoxic Function of Adoptive NK Cells. Blood, 128(22). Retrieved from http://www.bloodjournal.org/content/128/22/2160/tab-article-info
  • Otegbeye, F., Moreton, S., Ojo, E., Mackowski, N., Desai, A., Markowitz, S., & Wald, D. (2016). Enhancing Trafficking of Adoptively Transferred Natural Killer Cells Via Prostaglandin E2 Signaling. Biology of Blood and Marrow Transplantation, 22(3). doi:10.1016/j.bbmt.2015.11.1093, http://www.bbmt.org/article/S1083-8791(15)01850-9/fulltext
  • Ignatz-Hoover, J. J., Mackowski, N., & Wald, D. N. (2016). Abstract 1154: Elevated nuclear GSK3β promotes more aggressive disease in AML. Cancer Research Cancer Res, 76(14 Supplement), 1154-1154. doi:10.1158/1538-7445.am2016-1154, http://cancerres.aacrjournals.org/content/76/14_Supplement/1154
  • Shao Q, Casin KM, Mackowski N, Murphy E, Steenbergen C, et al. (2017). Adenosine A1 receptor activation increases myocardial protein S-nitrosothiols and elicits protection from ischemia-reperfusion injury in male and female hearts. PLOS ONE 12(5): e0177315. https://doi.org/10.1371/journal.pone.0177315, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177315
  • Otegbeye, Ojo, Moreton, Mackowski N., Lee, Lima, Wald. (2018) Inhibiting TGF-beta signaling preserves the function of highly activated, in vitro expanded natural killer cells in AML and colon cancer models. PLOS ONE 13(1): e0191358. https://doi.org/10.1371/journal.pone.0191358, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191358

PROFESSIONAL EXPERIENCE : KOHR LAB, JOHNS HOPKINS SCHOOL OF PUBLIC HEALTH, Baltimore, Maryland, 2016-2018 Research Technologist

  • Redox signaling pathways in the cardiovascular system, post translational protein modifications
  • Independent project on protein nitrosylation and cardioprotection
  • Performing and planning experiments with the purpose of uncovering the role of nitric oxide signaling during ischemia/reperfusion
  • Experiments included the use of protein purification, mass-spec, bioinformatic analysis of proteins, perfusing hearts ex-vivo to assess function, and transcription level analysis of regulatory genes
  • Managing laboratory operations including mouse colony

WALD LAB, CWRU SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, Cleveland, Ohio, 2014-2016 Research Technologist

  • Therapeutic strategies for Acute Myeloid Leukemia including immunotherapy and lessening the toxicity of existing small molecule drugs
  • Used mouse models xenografted with human AML cells to test a variety of similar compounds, as well as tested ways to improve therapeutic immune cell efficacy and culture
  • Maintained mouse colony including breeding and genotyping

SEQUENTIA, Cleveland, Ohio, 2014-2015 Business Development

  • Tested the online product to ensure proper function and ease of use
  • Analyzed hospital patient transfer data to understand how hospitals can improve efficiency
  • Drafted presentations to be given to potential clients
  • Studied different regional markets to see where our product would be most needed

WEISS LAB, CWRU SCHOOL OF MEDICINE, DEPARTMENT OF BIOCHEMISTRY, Cleveland, Ohio, 2012-2013 Undergraduate Research Assistant

  • Transcription factors involved in mammalian development
  • Created and prepared plasmid vectors for transfecting various gene fragments into rat cells
  • Maintained a mammalian cell tissue culture for transfection and analysis

INSTITUTE FOR THE SCIENCE OF ORIGINS, Cleveland, Ohio, 2011-­2013 Coordinator

  • Assisted with educational programs and research meetings with the purpose of advancing the scientific understanding of evolutionary and cosmological origins

U.S. SENATOR SHERROD BROWN (OH), Cleveland, Ohio, 2011-­2012 Intern

  • Assisted in meetings with constituents and organizations
  • Increased database efficiency and accuracy by compiling data for internal use
  • Managed calendar for Senator, kept organization

RESIDENTIAL HOUSING, CASE WESTERN RESERVE UNIVERSITY, Cleveland, Ohio, 2010-2011 Resident Assistant

  • Acted as a trained peer leader to student residents living in university housing
  • Completed administrative assignments, community building programs, and safety measures