Nathan Mackowski

EDUCATION : Johns Hopkins University

•  M.S. in Biotechnology student, expected graduation May 2018

Case Western Reserve University

• A. Evolutionary Biology & Political Science with minor in Russian Language, graduated in 2012
• Senior Political Science thesis: How NIH Grants are a Stimulus for Local Economies
• Winner of 2008 Common Reading Essay Contest

RELATED SKILLS :

PUBLICATIONS :

• Otegbeye, Mackowski, Ojo, Lima, & Wald. (2015). The IL-15 Super-Agonist ALT-803 Promotes Superior Activation and Cytotoxicity of Ex Vivo Expanded NK Cells Against AML. Blood,126(23), 3090. http://www.bloodjournal.org/content/126/23/3090?sso-checked=true
• Otegbeye, F., Mackowski, N., Ojo, E., Moreton, S., Lima, M. D., & Wald, D. (2016). Enhancing the Functional Activity of Natural Killer Cells Against Myeloid Leukemia with the IL-15 Super-Agonist, ALT-803. Biology of Blood and Marrow Transplantation, 22(3). doi:10.1016/j.bbmt.2015.11.485, http://www.bbmt.org/article/S1083-8791(15)01242-2/pdf
• Otegbeye, F., Ojo, E., Mackowski, N., Moreton, S., & Wald, D. (2016). Inhibiting TGF-Beta Signaling in the Tumor Micro-Environment Enhances the Cytotoxic Function of Adoptive NK Cells. Blood, 128(22). Retrieved from http://www.bloodjournal.org/content/128/22/2160/tab-article-info
• Otegbeye, F., Moreton, S., Ojo, E., Mackowski, N., Desai, A., Markowitz, S., & Wald, D. (2016). Enhancing Trafficking of Adoptively Transferred Natural Killer Cells Via Prostaglandin E2 Signaling. Biology of Blood and Marrow Transplantation, 22(3). doi:10.1016/j.bbmt.2015.11.1093, http://www.bbmt.org/article/S1083-8791(15)01850-9/fulltext
• Ignatz-Hoover, J. J., Mackowski, N., & Wald, D. N. (2016). Abstract 1154: Elevated nuclear GSK3β promotes more aggressive disease in AML. Cancer Research Cancer Res, 76(14 Supplement), 1154-1154. doi:10.1158/1538-7445.am2016-1154, http://cancerres.aacrjournals.org/content/76/14_Supplement/1154
• Shao Q, Casin KM, Mackowski N, Murphy E, Steenbergen C, et al. (2017). Adenosine A1 receptor activation increases myocardial protein S-nitrosothiols and elicits protection from ischemia-reperfusion injury in male and female hearts. PLOS ONE 12(5): e0177315. https://doi.org/10.1371/journal.pone.0177315, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177315
• Otegbeye, Ojo, Moreton, Mackowski N., Lee, Lima, Wald. (2018) Inhibiting TGF-beta signaling preserves the function of highly activated, in vitro expanded natural killer cells in AML and colon cancer models. PLOS ONE 13(1): e0191358. https://doi.org/10.1371/journal.pone.0191358, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191358

PROFESSIONAL EXPERIENCE : KOHR LAB, JOHNS HOPKINS SCHOOL OF PUBLIC HEALTH, Baltimore, Maryland, 2016-2018 Research Technologist

• Redox signaling pathways in the cardiovascular system, post translational protein modifications
• Independent project on protein nitrosylation and cardioprotection
• Performing and planning experiments with the purpose of uncovering the role of nitric oxide signaling during ischemia/reperfusion
• Experiments included the use of protein purification, mass-spec, bioinformatic analysis of proteins, perfusing hearts ex-vivo to assess function, and transcription level analysis of regulatory genes
• Managing laboratory operations including mouse colony

WALD LAB, CWRU SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, Cleveland, Ohio, 2014-2016 Research Technologist

• Therapeutic strategies for Acute Myeloid Leukemia including immunotherapy and lessening the toxicity of existing small molecule drugs
• Used mouse models xenografted with human AML cells to test a variety of similar compounds, as well as tested ways to improve therapeutic immune cell efficacy and culture
• Maintained mouse colony including breeding and genotyping

SEQUENTIA, Cleveland, Ohio, 2014-2015 Business Development

• Tested the online product to ensure proper function and ease of use
• Analyzed hospital patient transfer data to understand how hospitals can improve efficiency
• Drafted presentations to be given to potential clients
• Studied different regional markets to see where our product would be most needed

WEISS LAB, CWRU SCHOOL OF MEDICINE, DEPARTMENT OF BIOCHEMISTRY, Cleveland, Ohio, 2012-2013 Undergraduate Research Assistant

• Transcription factors involved in mammalian development
• Created and prepared plasmid vectors for transfecting various gene fragments into rat cells
• Maintained a mammalian cell tissue culture for transfection and analysis

INSTITUTE FOR THE SCIENCE OF ORIGINS, Cleveland, Ohio, 2011-­2013 Coordinator

• Assisted with educational programs and research meetings with the purpose of advancing the scientific understanding of evolutionary and cosmological origins

U.S. SENATOR SHERROD BROWN (OH), Cleveland, Ohio, 2011-­2012 Intern

• Assisted in meetings with constituents and organizations
• Increased database efficiency and accuracy by compiling data for internal use
• Managed calendar for Senator, kept organization

RESIDENTIAL HOUSING, CASE WESTERN RESERVE UNIVERSITY, Cleveland, Ohio, 2010-2011 Resident Assistant

• Acted as a trained peer leader to student residents living in university housing
• Completed administrative assignments, community building programs, and safety measures