A postdoc position is available at the Institut Pasteur in the signalling and host-parasite interactions group, headed by Dr Najma Rachidi, to investigate the subversion of host cell signalling by pathogens, using Leishmania infection as model.
A common feature of the most successful intracellular pathogens is the efficient manipulation of their host cell. Most molecular mechanisms governing the subversion of host cell signalling by pathogens involve release of pathogen-derived molecules into the host cell cytoplasm and direct interaction with host signalling proteins. Leishmania is a good model to study the impact of secreted parasite proteins on host cell phenotype, as well as their relevance for intracellular survival and disease development. In its mammalian host, Leishmania parasite, the causative agent of leishmaniasis, is phagocytosed by macrophages, but unlike other pathogens, survives and proliferates. Leishmania, through the release of effector molecules, subverts its host cell to insure its survival. One of these effectors particularly attracted our attention, Leishmania Casein Kinase 1, a signalling protein kinase, essential for intracellular parasite survival. Our data suggest that this kinase fulfils a significant part of its function in the macrophage, by regulating processes such as trafficking, translation or apoptosis; processes also modulated during Leishmania infection. Our data, which were confirmed in a cellular model, indicate that Leishmania Casein Kinase 1 is an important player in host-parasite interactions. The project, part of the ANR TEXLEISH, will combine genome editing, siRNA, cutting-edge microscopy and an innovative method to follow the dynamic of infection with the aim to reveal the mechanisms by which L-CK1.2 control host signalling pathways such as cell trafficking during Leishmania infection. For more details about our work, visit https://research.pasteur.fr/en/team/signalling-and-host-parasite-interactions/.
We are looking for a motivated post-doctoral researcher with a strong background in cell culture, gene knockout and knockdown (CRISPR Cas9, siRNA and ShRNA) in mammalian cells (cell lines and/or primary cells). Expertise in one of these two fields: proteomics/phospho-proteomics data analysis or imaging is not required but would be a bonus. The candidate should be able to work in a multi-disciplinary environment, have a solid record of scientific achievement and a decent level in English. The contract is for two years but the applicant will be strongly encouraged to apply for external and/or internal fellowships. The contract will start on the 30th of October 2023 at the latest.
Please send a cover letter, a CV with a publication list and three references to Najma Rachidi at firstname.lastname@example.org. Applications should be sent before the 30th of May 2023.