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© Biologie structurale et chimie
Structure du domaine en doigt de zinc de la protéine NEMO, déterminée par Résonance magnétique nucléaire (RMN). Cette protéine jouant un rôle dans des maladies (cancer, inflammation), les connaissances acquises sur sa structure offrent de précieuses informations sur sa fonction.
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Technique

Tecan • Spark

The Spark multimode microplate reader offers solutions for virtually any life science research or drug discovery  applications. The unique Fusion Optics allows any combination of filters or monochromators on both the excitation AND emission sides for every fluorescence measurement. This is complemented by the unparalleled multi-color luminescence module, which offers flexibility for virtually any luminescence measurement, including flash, glow, BRET and laser-based Alpha Technology. Spark’s Te-Cool™ module allows the user to set the reader temperature at, or even below, ambient for more accurate and robust results.

Applications:
• ELISAs
• Low-volume DNA/RNA quantification
• Nucleic acid labeling efficiency
• Protein quantification
• Reporter gene assays
• Homogeneous time-resolved fluorescence (HTRF®)
• Transcreener®
• Dual-Luciferase® Reporter (DLR™)
• Bioluminescence resonance energy
transfer (BRET) – including Nano-
BRET™

Detection modes:
• Absorbance
– including UV/vis spectra
• Fluorescence top and bottom
– including spectra
• Time-resolved fluorescence (TRF)
– including spectra
• Fluorescence Resonance Energy
Transfer (FRET)
• Time-resolved fluorescence
Resonance Energy Transfer
(TR-FRET)
• Fluorescence Polarization (FP)
• Luminescence – glow, flash,
multi-color, spectra
• AlphaScreen®, AlphaLISA® and
AlphaPlex™

For more information, visit : https://lifesciences.tecan.fr/multimode-plate-reader