The MoFlo Astrios is created by Beckman Coulter’s compagny. This is a jet-in-the-air sorter and has 5 or 7 lasers (UV, violet, red, blue and yellow-green) and two light scatter parameter can be measured at the same time. Seems it is in a BSL 2 safety cabinet, you have the possibility to sort P2 level cells. The standard nozzle size is 100 um and the pressure is set to 25 psi. This permits sorting from 1 up to 6 differents populations of cells with a rate of 100,000 particles per second.
We have recently developed the nanosorting approach for the detection, quantification of sub cellular elements.
This equipment is dedicated for assisted sorts sessions, please contact the team pfc@pasteur.fr
ASTERIX
- 5 lasers (355nm, 405nm, 488nm, 561nm and 638nm)
- This sorter is under a P2 PSM : P2 pathogens and Blood
- Nozzle sizes : 70um; 85um; 100um
- Sorting : 1 to 6 way for tubes and on plates 24, 96 and 384 wells.
- Software : Summit
- Astérix Optical Configuration
OBELIX
- 7 lasers (355nm, 405nm, 488nm, 532nm, 561nm, 592nm and 640nm)
- This sorter is under a P2 PSM : P2 pathogens and Blood
- SSC parameter available on 405 nm, 488 nm and 561 nm lasers
- Nozzle sizes : 70um; 85um; 100um
- Sorting : 1 to 6 way for tubes and on plates 24, 96 and 384 wells.
- Software : Summit
- Obélix Optical Configuration
