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© Christophe Thomas, Institut Pasteur
Gel d’électrophorèse lors de la purification d’un échantillon protéique
Publication : Diagn Microbiol Infect Dis

Development and comparative evaluation of SARS-CoV-2 S-RBD and N based ELISA tests in various African endemic settings

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Diagn Microbiol Infect Dis - 01 Apr 2023

Chaouki Benabdessalem, Wafa Ben Hamoudaa, Soumaya Marzoukia, Rokhaya Faye, Adji Astou Mbow, Babacar Diouf, Oumar Ndiaye, Ndongo Dia, Ousmane Faye, Amadou A. Sall, Cheikh Tidiane Diagne, Houda Amellal, Sayeh Ezzikouri, Diary Juliannie Ny Mioramalala, Fanirisoa Randrianarisaona, Khaled Trabelsi, Mohamed Boumaiza, Sonia Ben Hamouda, Rym Ouni, Soumaya Bchiri, Amani Chaaban, Mariem Gdoura, Yousr Gorgi, Imen Sfar, Sadok Yalaoui, Jalila Ben Khelil, Agnes Hamzaoui, Meya Abdallah, Yosra Cherif, Stephane Petres, Chris Ka Pun Mok, Nicolas Escriou, Sebastien Quesney, Koussay Dellagim, Matthieu Schoenhals, M’hammed Sarih, Ines Vigan-Womas, Jihene Bettaieb, Samia Rourou, Mohamed Ridha Barbouche, Melika Ben Ahmed

Link to Pubmed [PMID] – 36805620

Link to DOI – 10.1016/j.diagmicrobio.2023.115903

Diagn Microbiol Infect Dis . 2023 Apr;105(4):115903.

Management of the COVID-19 pandemic relies on molecular diagnostic methods supported by serological tools. Herein, we developed S-RBD- and N- based ELISA assays useful for infection rate surveillance as well as the follow-up of acquired protective immunity against SARS-CoV-2. ELISA assays were optimized using COVID-19 Tunisian patients’ sera and prepandemic controls. Assays were further validated in 3 African countries with variable endemic settings. The receiver operating curve was used to evaluate the assay performances. The N- and S-RBD-based ELISA assays performances, in Tunisia, were very high (AUC: 0.966 and 0.98, respectively, p < 0.0001). Cross-validation analysis showed similar performances in different settings. Cross-reactivity, with malaria infection, against viral antigens, was noticed. In head-to-head comparisons with different commercial assays, the developed assays showed high agreement. This study demonstrates, the added value of the developed serological assays in low-income countries, particularly in ethnically diverse populations with variable exposure to local endemic infectious diseases.

Keywords: COVID-19; ELISA; Endemic African settings; Multicentric validation; Nucleoprotein N; S-RBD.